![]() OTU abundance table can be further used to presume for metabolic functions. NMDS and PCoA for quantification of beta diversity (Jovel et al. The two most commonly used ordination tools are principal coordinate analyses (PCoA) and non-metric multidimensional scaling (NMDS).įigure 2. Once distances or dissimilarities between samples have been determined, they can be ordinated in a low-dimensional space to better illustrate how closely related they are to each other. There are two main methods for quantifying β diversity: phylogenetic β diversity that considers the evolutionary differences between communities (such as UniFrac), and non-phylogenetic or taxon-based methods (such as Bray-Curtis dissimilarity). There are two common normalization procedures: the total sum and upper quartile normalization. Before quantifying β diversity, the read counts (reads mapped to each taxon) must be normalized to minimize the technical variability between samples.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |